AT5G12290

Description : dgd1 suppressor 1

Gene families : OG_01_0004228 (OrthoFinder output from Ath, Cre, Mpo, Ppa and Zci) Phylogenetic Tree(s): OG0004228_tree

Sequence : coding (download), protein (download)

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Note:Only the main profile, including all conditions, is shown. Additional statistics and tissue specific profiles are available here.

Type	Description	Actions
Neighborhood	HRR: AT5G12290
Cluster	HCCA: Cluster_76

Type	GO Term	Name	Evidence	Source
BP	GO:0000902	cell morphogenesis	None	Extended
BP	GO:0000904	cell morphogenesis involved in differentiation	None	Extended
BP	GO:0003006	developmental process involved in reproduction	None	Extended
CC	GO:0005575	cellular_component	None	Extended
CC	GO:0005739	mitochondrion	IDA	Interproscan
CC	GO:0005741	mitochondrial outer membrane	IDA	Interproscan
BP	GO:0006464	cellular protein modification process	None	Extended
BP	GO:0006508	proteolysis	None	Extended
BP	GO:0006629	lipid metabolic process	None	Extended
BP	GO:0006643	membrane lipid metabolic process	None	Extended
BP	GO:0006664	glycolipid metabolic process	None	Extended
BP	GO:0006807	nitrogen compound metabolic process	None	Extended
BP	GO:0007015	actin filament organization	None	Extended
BP	GO:0007155	cell adhesion	RCA	Interproscan
BP	GO:0008150	biological_process	None	Extended
BP	GO:0008152	metabolic process	None	Extended
BP	GO:0008610	lipid biosynthetic process	None	Extended
BP	GO:0009058	biosynthetic process	None	Extended
BP	GO:0009247	glycolipid biosynthetic process	None	Extended
CC	GO:0009507	chloroplast	ISM	Interproscan
CC	GO:0009536	plastid	None	Extended
BP	GO:0009653	anatomical structure morphogenesis	None	Extended
BP	GO:0009887	animal organ morphogenesis	RCA	Interproscan
BP	GO:0009888	tissue development	RCA	Interproscan
BP	GO:0009987	cellular process	None	Extended
BP	GO:0010053	root epidermal cell differentiation	None	Extended
BP	GO:0010090	trichome morphogenesis	RCA	Interproscan
BP	GO:0010228	vegetative to reproductive phase transition of meristem	RCA	Interproscan
BP	GO:0010638	positive regulation of organelle organization	RCA	Interproscan
CC	GO:0016020	membrane	None	Extended
BP	GO:0016043	cellular component organization	None	Extended
BP	GO:0016926	protein desumoylation	RCA	Interproscan
BP	GO:0018193	peptidyl-amino acid modification	None	Extended
BP	GO:0018205	peptidyl-lysine modification	None	Extended
BP	GO:0019374	galactolipid metabolic process	None	Extended
BP	GO:0019375	galactolipid biosynthetic process	IMP	Interproscan
BP	GO:0019538	protein metabolic process	None	Extended
CC	GO:0019867	outer membrane	None	Extended
BP	GO:0021700	developmental maturation	None	Extended
BP	GO:0022414	reproductive process	None	Extended
BP	GO:0022610	biological adhesion	None	Extended
BP	GO:0030154	cell differentiation	None	Extended
CC	GO:0031090	organelle membrane	None	Extended
CC	GO:0031966	mitochondrial membrane	None	Extended
CC	GO:0031968	organelle outer membrane	None	Extended
BP	GO:0032502	developmental process	None	Extended
BP	GO:0033043	regulation of organelle organization	None	Extended
BP	GO:0033044	regulation of chromosome organization	RCA	Interproscan
BP	GO:0036211	protein modification process	None	Extended
BP	GO:0042743	hydrogen peroxide metabolic process	None	Extended
BP	GO:0043170	macromolecule metabolic process	None	Extended
CC	GO:0043226	organelle	None	Extended
CC	GO:0043227	membrane-bounded organelle	None	Extended
CC	GO:0043229	intracellular organelle	None	Extended
CC	GO:0043231	intracellular membrane-bounded organelle	None	Extended
BP	GO:0043412	macromolecule modification	None	Extended
BP	GO:0044237	cellular metabolic process	None	Extended
BP	GO:0044238	primary metabolic process	None	Extended
BP	GO:0044249	cellular biosynthetic process	None	Extended
BP	GO:0044255	cellular lipid metabolic process	None	Extended
BP	GO:0044260	cellular macromolecule metabolic process	None	Extended
BP	GO:0044267	cellular protein metabolic process	None	Extended
BP	GO:0045010	actin nucleation	RCA	Interproscan
BP	GO:0045229	external encapsulating structure organization	None	Extended
BP	GO:0046467	membrane lipid biosynthetic process	None	Extended
BP	GO:0048469	cell maturation	None	Extended
BP	GO:0048518	positive regulation of biological process	None	Extended
BP	GO:0048522	positive regulation of cellular process	None	Extended
BP	GO:0048764	trichoblast maturation	None	Extended
BP	GO:0048765	root hair cell differentiation	RCA	Interproscan
BP	GO:0048856	anatomical structure development	None	Extended
BP	GO:0048869	cellular developmental process	None	Extended
BP	GO:0050665	hydrogen peroxide biosynthetic process	IMP	Interproscan
BP	GO:0050665	hydrogen peroxide biosynthetic process	RCA	Interproscan
BP	GO:0050789	regulation of biological process	None	Extended
BP	GO:0050794	regulation of cellular process	None	Extended
BP	GO:0051128	regulation of cellular component organization	None	Extended
BP	GO:0051130	positive regulation of cellular component organization	None	Extended
BP	GO:0065007	biological regulation	None	Extended
BP	GO:0070646	protein modification by small protein removal	None	Extended
BP	GO:0070647	protein modification by small protein conjugation or removal	None	Extended
BP	GO:0071554	cell wall organization or biogenesis	None	Extended
BP	GO:0071555	cell wall organization	RCA	Interproscan
BP	GO:0071695	anatomical structure maturation	None	Extended
BP	GO:0071704	organic substance metabolic process	None	Extended
BP	GO:0071840	cellular component organization or biogenesis	None	Extended
BP	GO:0072593	reactive oxygen species metabolic process	None	Extended
BP	GO:0090627	plant epidermal cell differentiation	None	Extended
BP	GO:0097435	supramolecular fiber organization	None	Extended
CC	GO:0098588	bounding membrane of organelle	None	Extended
CC	GO:0110165	cellular anatomical entity	None	Extended
BP	GO:1901135	carbohydrate derivative metabolic process	None	Extended
BP	GO:1901137	carbohydrate derivative biosynthetic process	None	Extended
BP	GO:1901564	organonitrogen compound metabolic process	None	Extended
BP	GO:1901576	organic substance biosynthetic process	None	Extended
BP	GO:1903409	reactive oxygen species biosynthetic process	None	Extended
BP	GO:1903509	liposaccharide metabolic process	None	Extended

Toggle parental

Type	GO Term	Name	Evidence	Source
BP	GO:0000079	regulation of cyclin-dependent protein serine/threonine kinase activity	IEP	HCCA
BP	GO:0000098	sulfur amino acid catabolic process	IEP	HCCA
MF	GO:0000257	nitrilase activity	IEP	HCCA
BP	GO:0001709	cell fate determination	IEP	HCCA
BP	GO:0001932	regulation of protein phosphorylation	IEP	HCCA
BP	GO:0001933	negative regulation of protein phosphorylation	IEP	HCCA
MF	GO:0002161	aminoacyl-tRNA editing activity	IEP	HCCA
BP	GO:0002218	activation of innate immune response	IEP	HCCA
BP	GO:0002253	activation of immune response	IEP	HCCA
BP	GO:0002684	positive regulation of immune system process	IEP	HCCA
MF	GO:0003824	catalytic activity	IEP	HCCA
MF	GO:0003923	GPI-anchor transamidase activity	IEP	HCCA
MF	GO:0004028	3-chloroallyl aldehyde dehydrogenase activity	IEP	HCCA
MF	GO:0004335	galactokinase activity	IEP	HCCA
MF	GO:0004520	endodeoxyribonuclease activity	IEP	HCCA
MF	GO:0004536	deoxyribonuclease activity	IEP	HCCA
MF	GO:0004634	phosphopyruvate hydratase activity	IEP	HCCA
MF	GO:0004664	prephenate dehydratase activity	IEP	HCCA
MF	GO:0004708	MAP kinase kinase activity	IEP	HCCA
MF	GO:0004743	pyruvate kinase activity	IEP	HCCA
CC	GO:0005768	endosome	IEP	HCCA
CC	GO:0005771	multivesicular body	IEP	HCCA
CC	GO:0005794	Golgi apparatus	IEP	HCCA
CC	GO:0005802	trans-Golgi network	IEP	HCCA
BP	GO:0005975	carbohydrate metabolic process	IEP	HCCA
BP	GO:0005996	monosaccharide metabolic process	IEP	HCCA
BP	GO:0006040	amino sugar metabolic process	IEP	HCCA
BP	GO:0006063	uronic acid metabolic process	IEP	HCCA
BP	GO:0006094	gluconeogenesis	IEP	HCCA
BP	GO:0006096	glycolytic process	IEP	HCCA
BP	GO:0006165	nucleoside diphosphate phosphorylation	IEP	HCCA
BP	GO:0006308	DNA catabolic process	IEP	HCCA
BP	GO:0006400	tRNA modification	IEP	HCCA
BP	GO:0006469	negative regulation of protein kinase activity	IEP	HCCA
BP	GO:0006513	protein monoubiquitination	IEP	HCCA
BP	GO:0006558	L-phenylalanine metabolic process	IEP	HCCA
BP	GO:0006605	protein targeting	IEP	HCCA
BP	GO:0006625	protein targeting to peroxisome	IEP	HCCA
BP	GO:0006626	protein targeting to mitochondrion	IEP	HCCA
BP	GO:0006635	fatty acid beta-oxidation	IEP	HCCA
BP	GO:0006757	ATP generation from ADP	IEP	HCCA
BP	GO:0006777	Mo-molybdopterin cofactor biosynthetic process	IEP	HCCA
BP	GO:0006808	regulation of nitrogen utilization	IEP	HCCA
BP	GO:0006810	transport	IEP	HCCA
BP	GO:0006886	intracellular protein transport	IEP	HCCA
BP	GO:0006891	intra-Golgi vesicle-mediated transport	IEP	HCCA
BP	GO:0007006	mitochondrial membrane organization	IEP	HCCA
BP	GO:0007007	inner mitochondrial membrane organization	IEP	HCCA
BP	GO:0007031	peroxisome organization	IEP	HCCA
BP	GO:0007032	endosome organization	IEP	HCCA
BP	GO:0007041	lysosomal transport	IEP	HCCA
BP	GO:0008104	protein localization	IEP	HCCA
BP	GO:0008333	endosome to lysosome transport	IEP	HCCA
MF	GO:0008417	fucosyltransferase activity	IEP	HCCA
MF	GO:0008660	1-aminocyclopropane-1-carboxylate deaminase activity	IEP	HCCA
MF	GO:0009029	tetraacyldisaccharide 4'-kinase activity	IEP	HCCA
BP	GO:0009056	catabolic process	IEP	HCCA
BP	GO:0009062	fatty acid catabolic process	IEP	HCCA
BP	GO:0009093	cysteine catabolic process	IEP	HCCA
BP	GO:0009094	L-phenylalanine biosynthetic process	IEP	HCCA
BP	GO:0009095	aromatic amino acid family biosynthetic process, prephenate pathway	IEP	HCCA
BP	GO:0009132	nucleoside diphosphate metabolic process	IEP	HCCA
BP	GO:0009135	purine nucleoside diphosphate metabolic process	IEP	HCCA
BP	GO:0009179	purine ribonucleoside diphosphate metabolic process	IEP	HCCA
BP	GO:0009185	ribonucleoside diphosphate metabolic process	IEP	HCCA
BP	GO:0009245	lipid A biosynthetic process	IEP	HCCA
BP	GO:0009636	response to toxic substance	IEP	HCCA
BP	GO:0009692	ethylene metabolic process	IEP	HCCA
BP	GO:0009693	ethylene biosynthetic process	IEP	HCCA
BP	GO:0009749	response to glucose	IEP	HCCA
BP	GO:0009864	induced systemic resistance, jasmonic acid mediated signaling pathway	IEP	HCCA
BP	GO:0009866	induced systemic resistance, ethylene mediated signaling pathway	IEP	HCCA
BP	GO:0009969	xyloglucan biosynthetic process	IEP	HCCA
BP	GO:0010098	suspensor development	IEP	HCCA
BP	GO:0010351	lithium ion transport	IEP	HCCA
BP	GO:0010383	cell wall polysaccharide metabolic process	IEP	HCCA
BP	GO:0010410	hemicellulose metabolic process	IEP	HCCA
BP	GO:0010411	xyloglucan metabolic process	IEP	HCCA
BP	GO:0010417	glucuronoxylan biosynthetic process	IEP	HCCA
BP	GO:0010440	stomatal lineage progression	IEP	HCCA
BP	GO:0010563	negative regulation of phosphorus metabolic process	IEP	HCCA
MF	GO:0015020	glucuronosyltransferase activity	IEP	HCCA
BP	GO:0015031	protein transport	IEP	HCCA
MF	GO:0015095	magnesium ion transmembrane transporter activity	IEP	HCCA
MF	GO:0015386	potassium:proton antiporter activity	IEP	HCCA
BP	GO:0015919	peroxisomal membrane transport	IEP	HCCA
BP	GO:0016042	lipid catabolic process	IEP	HCCA
BP	GO:0016050	vesicle organization	IEP	HCCA
BP	GO:0016054	organic acid catabolic process	IEP	HCCA
BP	GO:0016192	vesicle-mediated transport	IEP	HCCA
MF	GO:0016278	lysine N-methyltransferase activity	IEP	HCCA
MF	GO:0016279	protein-lysine N-methyltransferase activity	IEP	HCCA
BP	GO:0016482	cytosolic transport	IEP	HCCA
BP	GO:0016485	protein processing	IEP	HCCA
BP	GO:0016558	protein import into peroxisome matrix	IEP	HCCA
MF	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors	IEP	HCCA
MF	GO:0016615	malate dehydrogenase activity	IEP	HCCA
MF	GO:0016740	transferase activity	IEP	HCCA
MF	GO:0016757	glycosyltransferase activity	IEP	HCCA
MF	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	IEP	HCCA
MF	GO:0016815	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in nitriles	IEP	HCCA
MF	GO:0016829	lyase activity	IEP	HCCA
MF	GO:0016836	hydro-lyase activity	IEP	HCCA
MF	GO:0016846	carbon-sulfur lyase activity	IEP	HCCA
MF	GO:0016847	1-aminocyclopropane-1-carboxylate synthase activity	IEP	HCCA
BP	GO:0017038	protein import	IEP	HCCA
MF	GO:0018024	histone-lysine N-methyltransferase activity	IEP	HCCA
MF	GO:0019148	D-cysteine desulfhydrase activity	IEP	HCCA
BP	GO:0019319	hexose biosynthetic process	IEP	HCCA
BP	GO:0019395	fatty acid oxidation	IEP	HCCA
BP	GO:0019447	D-cysteine catabolic process	IEP	HCCA
BP	GO:0019478	D-amino acid catabolic process	IEP	HCCA
BP	GO:0019586	galacturonate metabolic process	IEP	HCCA
BP	GO:0019720	Mo-molybdopterin cofactor metabolic process	IEP	HCCA
BP	GO:0019752	carboxylic acid metabolic process	IEP	HCCA
MF	GO:0022821	potassium ion antiporter activity	IEP	HCCA
BP	GO:0030002	cellular anion homeostasis	IEP	HCCA
MF	GO:0030151	molybdenum ion binding	IEP	HCCA
BP	GO:0030258	lipid modification	IEP	HCCA
BP	GO:0030643	cellular phosphate ion homeostasis	IEP	HCCA
CC	GO:0030904	retromer complex	IEP	HCCA
BP	GO:0031400	negative regulation of protein modification process	IEP	HCCA
CC	GO:0031410	cytoplasmic vesicle	IEP	HCCA
CC	GO:0031982	vesicle	IEP	HCCA
CC	GO:0031984	organelle subcompartment	IEP	HCCA
BP	GO:0032269	negative regulation of cellular protein metabolic process	IEP	HCCA
BP	GO:0032787	monocarboxylic acid metabolic process	IEP	HCCA
MF	GO:0032942	inositol tetrakisphosphate 2-kinase activity	IEP	HCCA
BP	GO:0033036	macromolecule localization	IEP	HCCA
BP	GO:0033365	protein localization to organelle	IEP	HCCA
BP	GO:0033673	negative regulation of kinase activity	IEP	HCCA
BP	GO:0034440	lipid oxidation	IEP	HCCA
BP	GO:0034613	cellular protein localization	IEP	HCCA
MF	GO:0035252	UDP-xylosyltransferase activity	IEP	HCCA
MF	GO:0035299	inositol pentakisphosphate 2-kinase activity	IEP	HCCA
BP	GO:0042147	retrograde transport, endosome to Golgi	IEP	HCCA
BP	GO:0042278	purine nucleoside metabolic process	IEP	HCCA
MF	GO:0042285	xylosyltransferase activity	IEP	HCCA
BP	GO:0042325	regulation of phosphorylation	IEP	HCCA
BP	GO:0042326	negative regulation of phosphorylation	IEP	HCCA
BP	GO:0042546	cell wall biogenesis	IEP	HCCA
BP	GO:0043086	negative regulation of catalytic activity	IEP	HCCA
BP	GO:0043436	oxoacid metabolic process	IEP	HCCA
BP	GO:0043449	cellular alkene metabolic process	IEP	HCCA
BP	GO:0043450	alkene biosynthetic process	IEP	HCCA
BP	GO:0043545	molybdopterin cofactor metabolic process	IEP	HCCA
BP	GO:0043549	regulation of kinase activity	IEP	HCCA
BP	GO:0043574	peroxisomal transport	IEP	HCCA
BP	GO:0044036	cell wall macromolecule metabolic process	IEP	HCCA
BP	GO:0044092	negative regulation of molecular function	IEP	HCCA
BP	GO:0044242	cellular lipid catabolic process	IEP	HCCA
BP	GO:0044248	cellular catabolic process	IEP	HCCA
BP	GO:0044282	small molecule catabolic process	IEP	HCCA
BP	GO:0044743	protein transmembrane import into intracellular organelle	IEP	HCCA
BP	GO:0045039	protein insertion into mitochondrial inner membrane	IEP	HCCA
BP	GO:0045089	positive regulation of innate immune response	IEP	HCCA
BP	GO:0045184	establishment of protein localization	IEP	HCCA
BP	GO:0045736	negative regulation of cyclin-dependent protein serine/threonine kinase activity	IEP	HCCA
BP	GO:0045786	negative regulation of cell cycle	IEP	HCCA
BP	GO:0045859	regulation of protein kinase activity	IEP	HCCA
BP	GO:0045936	negative regulation of phosphate metabolic process	IEP	HCCA
BP	GO:0046031	ADP metabolic process	IEP	HCCA
BP	GO:0046034	ATP metabolic process	IEP	HCCA
BP	GO:0046128	purine ribonucleoside metabolic process	IEP	HCCA
BP	GO:0046364	monosaccharide biosynthetic process	IEP	HCCA
BP	GO:0046395	carboxylic acid catabolic process	IEP	HCCA
BP	GO:0046396	D-galacturonate metabolic process	IEP	HCCA
BP	GO:0046416	D-amino acid metabolic process	IEP	HCCA
BP	GO:0046438	D-cysteine metabolic process	IEP	HCCA
BP	GO:0046493	lipid A metabolic process	IEP	HCCA
BP	GO:0046835	carbohydrate phosphorylation	IEP	HCCA
BP	GO:0046907	intracellular transport	IEP	HCCA
BP	GO:0046939	nucleotide phosphorylation	IEP	HCCA
MF	GO:0046976	histone methyltransferase activity (H3-K27 specific)	IEP	HCCA
MF	GO:0047560	3-dehydrosphinganine reductase activity	IEP	HCCA
MF	GO:0047769	arogenate dehydratase activity	IEP	HCCA
MF	GO:0047912	galacturonokinase activity	IEP	HCCA
BP	GO:0048193	Golgi vesicle transport	IEP	HCCA
BP	GO:0050778	positive regulation of immune response	IEP	HCCA
BP	GO:0051189	prosthetic group metabolic process	IEP	HCCA
BP	GO:0051204	protein insertion into mitochondrial membrane	IEP	HCCA
BP	GO:0051205	protein insertion into membrane	IEP	HCCA
BP	GO:0051248	negative regulation of protein metabolic process	IEP	HCCA
BP	GO:0051338	regulation of transferase activity	IEP	HCCA
BP	GO:0051348	negative regulation of transferase activity	IEP	HCCA
MF	GO:0051499	D-aminoacyl-tRNA deacylase activity	IEP	HCCA
BP	GO:0051641	cellular localization	IEP	HCCA
BP	GO:0051649	establishment of localization in cell	IEP	HCCA
MF	GO:0051765	inositol tetrakisphosphate kinase activity	IEP	HCCA
BP	GO:0055062	phosphate ion homeostasis	IEP	HCCA
BP	GO:0065002	intracellular protein transmembrane transport	IEP	HCCA
BP	GO:0070585	protein localization to mitochondrion	IEP	HCCA
BP	GO:0070727	cellular macromolecule localization	IEP	HCCA
BP	GO:0070734	histone H3-K27 methylation	IEP	HCCA
BP	GO:0071702	organic substance transport	IEP	HCCA
BP	GO:0071705	nitrogen compound transport	IEP	HCCA
BP	GO:0071806	protein transmembrane transport	IEP	HCCA
BP	GO:0071900	regulation of protein serine/threonine kinase activity	IEP	HCCA
BP	GO:0071901	negative regulation of protein serine/threonine kinase activity	IEP	HCCA
BP	GO:0072329	monocarboxylic acid catabolic process	IEP	HCCA
BP	GO:0072502	cellular trivalent inorganic anion homeostasis	IEP	HCCA
BP	GO:0072506	trivalent inorganic anion homeostasis	IEP	HCCA
BP	GO:0072521	purine-containing compound metabolic process	IEP	HCCA
BP	GO:0072594	establishment of protein localization to organelle	IEP	HCCA
BP	GO:0072655	establishment of protein localization to mitochondrion	IEP	HCCA
BP	GO:0072662	protein localization to peroxisome	IEP	HCCA
BP	GO:0072663	establishment of protein localization to peroxisome	IEP	HCCA
MF	GO:0080116	glucuronoxylan glucuronosyltransferase activity	IEP	HCCA
BP	GO:0080171	lytic vacuole organization	IEP	HCCA
BP	GO:0080179	1-methylguanosine metabolic process	IEP	HCCA
BP	GO:0090151	establishment of protein localization to mitochondrial membrane	IEP	HCCA
CC	GO:0097708	intracellular vesicle	IEP	HCCA
CC	GO:0098791	Golgi apparatus subcompartment	IEP	HCCA
BP	GO:0120251	hydrocarbon biosynthetic process	IEP	HCCA
BP	GO:0120252	hydrocarbon metabolic process	IEP	HCCA
BP	GO:0120254	olefinic compound metabolic process	IEP	HCCA
BP	GO:0120255	olefinic compound biosynthetic process	IEP	HCCA
BP	GO:1900673	olefin metabolic process	IEP	HCCA
BP	GO:1900674	olefin biosynthetic process	IEP	HCCA
BP	GO:1901068	guanosine-containing compound metabolic process	IEP	HCCA
BP	GO:1901269	lipooligosaccharide metabolic process	IEP	HCCA
BP	GO:1901271	lipooligosaccharide biosynthetic process	IEP	HCCA
BP	GO:1901575	organic substance catabolic process	IEP	HCCA
BP	GO:1902221	erythrose 4-phosphate/phosphoenolpyruvate family amino acid metabolic process	IEP	HCCA
BP	GO:1902223	erythrose 4-phosphate/phosphoenolpyruvate family amino acid biosynthetic process	IEP	HCCA
BP	GO:1904029	regulation of cyclin-dependent protein kinase activity	IEP	HCCA
BP	GO:1904030	negative regulation of cyclin-dependent protein kinase activity	IEP	HCCA
BP	GO:1990019	protein storage vacuole organization	IEP	HCCA
BP	GO:2001289	lipid X metabolic process	IEP	HCCA

InterPro domains	Description	Start	Stop
IPR013946	NCA2	318	592

External Links

PLAZA 3.0 Dicots	AT5G12290

AT5G12290

Expression Profile

Co-expression Networks

Functional Annotation